Test of Kleihauer

Sometimes called in certain countries test of Kleihauer-Betke , this test of laboratory makes it possible to highlight the presence of Hématie S fetal among maternal adult red blood corpuscles.

This test is based on the resistance of the fetal Hémoglobine (Hb F) to a acid Solution. Adult hemoglobin (Hb A), less resistant, is éluée adult red blood corpuscles of which there remain only empty stromas with the reading under the microscope, whereas the fetal red blood corpuscles remain coloured.

The rate of the fetal red blood corpuscles (which contain hemoglobin F) compared to the maternal adult red blood corpuscles (which contain hemoglobin A) makes it possible to estimate the volume of fetal blood present in maternal circulation. This rate is given of many fetal red blood corpuscles for 10.000 adult red blood corpuscles (HF/10000 ha).

This estimate makes it possible to adapt the quantity of Immunoglobuline S anti-D to inject into the case of the birth of a child RH+ at a woman RH. This injection prevents the hello-immunization of the mother, and avoids the haemolytic Maladie of the newborn RH1-D at the time of the following pregnancy.

Also sometimes this estimate makes it possible to identify the cause of a fetal Anémie, or to appreciate the fetal participation in a uterine Hémorragie during a pregnancy, in the event of Placenta praevia for example.

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