TAE

The TAE is a Buffer solution of migration used in electrophoresis on Gel of agarose of the Nucleic acid . It is composed of Tris, Acétate and EDTA, generally with the pH 8,0.

Concentration

  • TBE 50x : Solution concentrated mother fifty times, to dilute to obtain the desired concentration.
  • TAE 10x : Solution concentrated mother ten times, to dilute to obtain the desired concentration.
  • TAE 1x : solution lends to employment.

preparation

For the preparation of a plug TAE (50x) pH 8,0 to weigh:
  • 242 G TrisBase,
  • 57,1 crystalline acetic acid ml 100%,
  • 100 ml EDTA 0,5M.

To put in a graduated flask and to put the sufficient quantity for 1000 ml of distilled water.

See too

TBE

Random links:Mendes handbook | Route main road 563 | Trazodone | ☼ | Peeled Vitor | IBM_WebExplorer