Safety in laboratory

During his work, the personnel of laboratory exposes itself to Danger S:

chemical, biological, radiological, physical and other

These dangers can be limited, if the fundamental safety regulations are complied with. In a laboratory, it is necessary to adopt an attitude reflected not to endanger its life and the life of others.

General rules of safety in a laboratory

Obligations

To locate the sites of the materials of safety (showers, Extinguishing S,…).
To carry a blouse in Cotton and not in Polyester (cotton burns in the event of contact with a flame, whereas the polyester melts and adheres to the skin).
To withdraw all its jewels, not to carry make-up.
To attach the hair
To carry the glasses
to wash the hands while entering and while leaving the laboratory.
nothing to let trail on the ground or on the straw mattresses.
To arrange the material as soon as it is not necessary any more in order not to be constrained during your next handling, to also learn how to manage the workspace and the time one has.
All the bottles must without exception have a label on which one finds the name, the formula, it (S) pictogram (S) of safety, the sentences R and S.
Lire the instructions of a material or a bottle the commercial
Vérifier the material out of glass before use (to eliminate any glass blowpipe, spangled,…).
to refer to the pictograms when they are present and the sentences R and S.
Mettre dangerous bottles in protected rooms

To reflect before acting.

Prohibitions

to smoke, drink, or eat in a laboratory.
Of pipetter to the mouth any chemicals.
to throw to the sink of the chemicals, biological or radioactive.
to handle a flammable product near a flame or of a hot spot.
to run
to handle without glasses and blouse

Chemical dangers

The collective protective gears must be used before the individual protective gears. Indeed, the port of the mask in laboratory protects the person who handles but does not protect the other people present in the laboratory. The work confined under a range hood removes the painfulness of the port of the mask and protects the others.

The person handling of the bottles containing of the products commercial is exposed constantly with the chemical risks. Before any handling of a bottle, it is appropriate of:

To carry the adapted protective gears: Blouse out of cotton, approved safety goggles the EEC, protective gloves.
the handling of the bottles is done under ventilated fume cupboard (for the acids or bases concentrated for example).
To check the glassmaking before any handling to avoid cuts (notched material) or breaks material in the course of use (notched glassmaking, glares,…)
Always to put a vat of retention under the nonempty engines, bottles,… in order not to spread of liquid in the event of incident with the material
not to be used itself for the bottle. Transvase in a large clean beaker and annotate the volume which you need for your handling. That will avoid you finding you with solutions " polluées".
Refer to the pictograms:

Biological dangers

This pictogram is present:

at the entry of a laboratory of microbiology or biology, like on the containers intended to receive waste (Boxes of plastic Petri, Pasteur pipette, tubes with hémolyses…) or of the biological products (taking away ESB, blood,…).
it means the contamination propable by biological agent of nature known or not (bacteria, protozoa, virus, let us request…).
with the sight of this symbol, comply with the rules of asepsis, to wash the hands with water and the soap after handling.
in the event of accident (discharge on the straw mattress, cut with a contaminated material,…) to wash and disinfect the wound and/or the scheme of work.
To protect the wounds by vinyl or latex gloves.
To hold the notebook of up to date vaccination.

Biological risk

Radiological risk

Shrinking of the gloves

As we saw previously, the gloves are very useful to protect the hands, however protection is null if you do not know how to withdraw them:

to fold the wrist of a hand downwards.
with the other hand, to grip the end of the glove.
to take off the glove to the phalanges, you must see the inner face being turned over on the external face.
to fold the second wrist and to completely take off the glove with the fingers still protected from the second hand.
to get rid of the last glove by making it fall while moving the fingers.

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