Plug phosphates saline

The plug phosphates saline (often shortened “PBS”, coming from English out of phosphate buffered saltworks ) is a Buffer solution usually used in biochemistry. It is about a physiological aqueous solution containing of the Sodium chloride, the trisodium Phosphate and the Phosphate of potassium. In general, the concentration of these salts is that of the human body (isotonicity). This plug is especially used to rinse the cells when it is necessary to remove any trace of medium before treating them. It is planned for a use on those maintained out of the incubator with CO2 is not conceived for long-term incubations.

Preparation

To prepare 1000mL PBS:

For certain uses, one adds to this plug of the Calcium (CaCl2) or of the Magnésium (MgCl2).

Applications

The plug phosphates has many applications of share its isotonicity and its not-toxicity. It can be used like Solvant, and solution of rinsing. Like it structure water around biomolécules (enzymatic proteins, proteins, etc) immobilized on solid surfaces, the plug phosphate can also be used like Diluant to ensure the dry storage of those. These thin layers of water make it possible to prevent the denaturation of the biomolécules, or the conformational changes. It is possible to use a Tampon carbonates to provide the same function, but those are less effective. The plug phosphate can also be used as spectrum of reference in ellipsometry during measurement of adsorption of a protein.

Additives make it possible to extend its field of application. Thus, a mixture of EDTA and PBS make it possible to detach from the piled up cells. On the other hand, its use with divalent metals can cause a precipitate. For this kind of use, a Tampon of Good is adapted often more.

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