Peptide coupling

A peptide reaction of coupling is a reaction of synthesis at the protein laboratory, from derived from amino-acid.

Principle

The reagent not having the same selectivity as the natural mechanisms, of protections of functions are néccessaires. The amino-acids are added the ones following the others, but after each addition a déprotection is necessary. In order to limit the risks of racemization, the coupling is generally made on the N-final end of peptide. A peptide reagent of coupling is used to allow the formation of the bond amide.

Reagents of peptide coupling

Liquid synthesis phase

Synthesis on solid face

The first N-protected amino-acid is acroché by the carboxylic acid function on a functionalized resin. A rinsing makes it possible to eliminate excess from reagents. Then the protective grouping of the amine terminal is cleaved, operation followed by a rinsing. A second N-protected amino-acid is added (in excess), as well as reagent of coupling. Once the reaction considered finished, one carries out a rinsing, then a déprotection. One continues the cycle to introduce all the amino-acids. At the end of the process, peptide is cleaved support.

See too

Amide

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