PALCAM (gélose)
PALCAM is a culture medium of Listeria monocytogenes .
Composition
- nutritive Agents:
- Peptone: 23,0 g/l
- Starch: 1,0 g/l
- Sodium chloride: 5,0 g/l
- Agar-agar (Columbia agar): 13,0 g/l
- D (+) - Mannitol: 10,0 g/l
- Ammonium iron-III-citrate: 0,5 g/l
- Esculine : 0,8 g/l
- Glucose: 0,5 g/l
- demineralized Water: 500 ml
- distilled Water sterilized: 1 ml
-
inhibiting Agents:
- Chloride lithium: 15,0 g/l
- Sulfate of polymyxine-B: 5 Mg in 500 ml
- Ceftazidime: 10,0 Mg in 500 ml
- Acriflavine: 2,5 Mg in 500 ml
-
Indicator:
- Red of phenol: 0,08 g/l
pH = 7,0 ± 0,2
Reading
Green colonies olives with black halation of diameter 1,5 Misters.
See too
- Culture medium
- List of culture media
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