Nitrite (bubble)

determination of the respiratory type of the micro-organisms.

Composition

  • infusion heart-brain: 25,0 G
  • nitrite of sodium: 10,0 G
  • pH = 7,2

Preparation

35 G per liter. Traditional pressure-sealing. The nitrite solution sterilized by filtration can also be added right before employment.

Reading

After having checked the presence of culture, to add the reagents Nitrite 1 and 2. A red coloring signs the presence of nitrites: bacterium nitrite réductase -. The absence of coloring signs the reduction by the bacterium of nitrogen nitrites. The addition of a bell or gélose allows the description of gas.

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