Muller-Kauffmann (basic bubble with the tétrathionate)

Bubble of enrichment in Salmonella

Composition

tryptone: 7,0 G
peptone of soya: 2,3 G
bile of ox: 4,8 G
green shining: 9,5 Mg
sodium chloride: 2,3 G
thiosulfate: 24,0 G
tétrathionate: 6,7 G
sodium: 11,8 G
potassium: 1,15 G
iodides: 7,4 G
iodine: 0,0 G
carbonate of calcium: 25,0 G
pH = 7,3

Preparation

82 G of basic powder are dissolved by boiling. One adds then 20 ml of an iodo-iodized solution (250 G IK and 200 G of Iodine by dm3 prepared by dissolving iodine in iodide) One adds finally 9,5 ml of a solution of green shining to 1 G by dm3.

Reading

The composition given here is calculated according to the additions of molecules reacting between them (Iodine on thiosulfate). This medium allows enrichment in Salmonella (but not in Shigella) by deceleration of the growth of the other bacteria compared to Salmonella.

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