Mueller-Hinton (Gélose)

Rich Gélose for the realization of the Antibiogramme standard

Composition

  • infusion of beef: 300,0 ml
  • peptone of casein: 17,5 G
  • starch of corn: 1,5 G
  • agar: 17,0 G
  • pH = 7,4

Preparation

38 G per liter. Sterilization with the autoclave. To prepare this medium it is necessary to weigh powder 38g and to mix it in 1L water. It is necessary to homogenize then to heat while agitating. It is necessary to carry to boiling during approximately a minute. Then it is necessary to sterilize the gélose with the autoclave during 15 minutes with 116°C.

Reading

This gélose standardized is the gélose making it possible to test the action of antibiotics on the bacteria. It can be added with blood (for Streptococcus), of globular extract (for Haemophilus), It must be cast out of box in order to obtain a thickness of 4 Misters It exists an equivalent bubble.

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