Mannitol-Mobility-Nitrate

Use

use of the Mannitol, reduction of the Nitrate S, semi-soft mobility in gélose

Composition

- hydrolysat tryptic of Casein: .................. 10,0 G - Mannitol: .......................................... 7,5 G - phenol red: ................................... 0,4 Mg - Nitrate of potassium: .............................. 1,0 G - agar: .............................................. 3,5 G pH = 7,6

Preparation

22 G per liter. Traditional sterilization.

Reading

  • a: not of degradation of the manitol

  • b: Degradation of the manitol
  • C: Degradation of the manitol with production of gas
  • D: Nonmobile bacterium, colonies instead of sowing
  • E: Mobile bacterium, distribution of the colonies in the medium

  • yellow medium: mannitol +

  • red medium: mannitol -
For a strict aerobic bacterium, the culture on all the height possibly accompanied by bubbles shows a breathing nitrates. The reduction of nitrates could be visualized by addition of the usual reagents. Their acidity involves a progressive turn with the yellow of a red medium: a red coloring after addition of nitrite 1 and 2 watch thus well the presence of nitrites.

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