MRS (Gélose de Man, Rogosa, Sharpe)

Use

culture of the lactic bacteria

Composition

  • peptone 10,0 G
  • beef extract 8,0 G
  • extracts from yeast 4,0 G
  • Glucose 20,0 G
  • Acétate from sodium trihydrate 5,0 G
  • Citrate ammonium 2,0 G
  • Tween 80 1,0 ml
  • hydrogénophosphate from potassium 2,0 G
  • Epsom salt heptahydraté 0,2 G
  • sulfate from manganese tétrahydraté 0,05 G
  • Agar 10,0 G
  • pH = 6,2

Preparation

62 G by litreSterilisation with the autoclave.

Reading

Medium allowing a good culture of the Lactobacillus. There exists an equivalent bubble.

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