Litsky (medium of)

Use

confirmation of Enterococcus (food Microbiology)

Composition

  • Peptone 20,0 G
  • Glucose 5,0 G
  • azide 0,2 G
  • éthyl-purple 0,5 G
  • NaCl 5,0 G
  • hydrogénophosphate of potassium 2,7 G
  • dihydrogénophosphate of potassium 2,7 G
  • pH = 6,8

Preparation

35,7 G per liter. Traditional pressure-sealing

Reading

This medium allows enrichment in Entérocoques of a inoculum of food product. A disorder signs the possible presence of these bacteria which it will then be necessary to confirm by the test of Litsky, the insulation and the identification of the colonies. It is used for the enumeration of Streptocoques fecal. It is a confirmative test which is done following the presumptive test in the middle of Rothe The reading of the tube is done thanks to the formed disorder and the possible formation of a pastille violet.

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