Lactosée in Tergitol 7 and the including all taxes (Gélose)
Use
It is a medium of research and enumeration of the coliformes. It is especially used for the colorimetry of water by the method of filtration.
Composition
- peptone 10,0 G
- beef extract 5,0 G
- extracts from yeast 6,0 G
- Lactose 20,0 G
- tergitol 7 10 Mg
- including all taxes 25 Mg
- Bleu from bromothymol 50 Mg
- agar 13,0 G
- pH = 7,2
Preparation
54,15 G per liter. Traditional sterilization. The tergitol is added in the medium in superfusion beforehand cooled to 45°C.
Reading
blue or green colonies: lactose - .colonies yellow: lactose +La reduction of the including all taxes in red compound is weak with Escherichia coli and Enterobacter aerogenes which thus give colonies yellow whereas those of the others coliformes are pink-reds and surrounded by a yellow halation. The tergitol 7 limit invasion by Proteus
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