King B

The King B is a Culture medium which allows the culture of to be Unicellulaire.

Use

Description of the Pyoverdine of Pseudomonas of the Fluorescent group.

Composition

  • peptone known as " B" 20,0 G
  • glycerol 10,0 G
  • hydrogénophosphate of potassium 1,5 G
  • Epsom salt heptahydraté 1,5 G
  • agar purified 12,0 G
  • pH = 7,2

Preparation

37 G of powder per liter. Traditional sterilization. To add 10 Cm3 of glycerol after pressure-sealing.

Reading

The Pyoverdine yellows the medium. This molecule presents a Fluorescence to 340 Nm. It is insoluble in the trichlorométhane (Chloroforme).

to see too

Random links:Pont de Wien | Hippodrome of Cano | Hezbollah | Price Oréal-UNESCO for the women and science | Providentissimus Deus | Ephor