In situ hybridization

See also: Hybridization, In situ

The in situ Hybridation (HIS) is a technique of laboratory to locate a known nucleic sequence mono-bit (ARN or DNA) on a histological Coupe fabric.

This technique uses the complementarity of the nitrogenized Bases between them, indeed if two complementary mono-bits are placed, they naturally will approach to form a propeller.

Thus to be able to locate molecule of DNA or ARN, one must to denature it while heating it (double bit => mono-bit), as well as probe used (molecule complementary to that which one seeks) and finally to mark the probe.

Markings

One can mark the probe with:

of the radioactive elements like the Tritium H ³, P³² or S³⁵ that one raised by Autoradiography

of the fluorescent products; Microscopy with fluorescence

of the Hapten S; biotine or dioxygénine révélable with avidine or streptavidine, or with Antibodies marked by a Enzyme

of the Enzyme S révélables with Antibody.

His is also realizable in electronic microscopy by using a marking with colloidal gold with several sizes of possible grains (0.8 to 20 Nm).

Related article

fluorescent Hybridization in-situ

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