Immunohistochimy

The immunohistochimy is the process of detection of Antigène S in fabrics by means of Anticorps. The antibodies can be of monoclonal origin polyclonale or , the monoclonal antibodies being more specific essentially. The immunohistochimy is largely used for the diagnosis and/or the follow-up of Cancer S. Of the specific markers is known for various cancers.

Methods

Antigen S in tissue, the direct and the indirect method. -->

Antibody (i.e FITC conjugated Antiserum) reacting directly with the Antigen in Tissue sections. Technical This utilizes only one Antibody and the procedure is therefore shorts and quick. However, it is insensitive due to little indirect signal amplification and rarely used since the introduction off the method.


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Antigen, and has labeled secondary antibody (second to bush-hammer) which reacts with the primary antibody. (The secondary antibody must Be against the animal IgG off the species in which the primary antibody has been raised.) This method is more sensitive due to different signal amplification through several secondary antibody reactions with antigenic sites one the primary antibody. The second to bush-hammer antibody edge Be labeled with has fluorescent dye such ace FITC, Rhodamine gold Texas red, and this is called the indirect Immunofluorescence method . The second to bush-hammer antibody may Be labeled with year Enzyme such ace Peroxidase, Alkaline phosphatase gold Glucose oxidase, and this is called the indirect Immunoenzyme method . Offering has substrate to thesis enzymes will demonstrate presence off the enzyme (and hence the antibody) in has quantifiable fashion. -->

External bonds

  • Immunohistochemical Staining Protocols
  • Immunohistochemistry Image Gallery
  • Immunohistochemistry Protocol Database

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