Hugh and Leifson

Use

determination of the way of attack of glucids

Composition

  • tryptone 2,0 G
  • blue of bromothymol 30 Mg
  • sodium chloride 5,0 G
  • hydrogénophosphate of potassium 0,3 G
  • agar 2,5 G
  • pH = 7,1

Preparation

9,8 G per liter. Traditional pressure-sealing. The glucid to be tested is to add before or after pressure-sealing to the final concentration of 10 G per liter.

Reading

This medium is used for the description of the respiratory type. The observation of the possible acidification on all the height of the tube sown in central puncture makes it possible to conclude. The sowing of two tubes of which one covered with sterile petroleum jello is useless. Certain bacteria (anaerobic strict, hulls Gram-plus) cultivate badly on this medium: CTA then will be preferred to him.

to see too

  • Research of the way of attack of the glucose
  • Culture medium
  • Culture media (list)

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