Fuchsine

The fuchsine is a purplished coloring red.

This product intervenes in the Coloration of Gram , but also in the bacterial culture media. In very an other field, the fuschine is also used to make layouts with the skin (also called “tattooings”) persistent in particular in external radiotherapy.

This substance was patented by the company Renard Brothers in the middle of the 19th century. The name of “fuchsine” is derived from Fuchs translation of “German fox”. This name evokes also the color close to that of the flowers of Fuchsia.

There exist three types of fuchsines.

Basic fuchsine

Basic néo-fuchsine

It is used in the fuchsine of Ziehl (Coloration of Gram, Coloration of Ziehl-Neelsen) or the fuchsine of Kinyoun (coloring of Kinyoun). Its structure is presented in figure 1.

Composition of the fuchsine of Ziehl - Basic Fuchsine ................. 10 G - Phenol ........................... 50 G - Ethanol .......................... 100 ml - Water distilled .................... 1 L

Composition of the fuchsine of Kinyoun - Basic Fuchsine ................. 33,3 G - Phenol ........................... 66,6 G - Ethanol .......................... 166 ml - Water distilled .................... 1 L

Acid fuchsines

They are colorless. Indeed, they are leuco bases (càd white derivatives) of basic fuchsine obtained by reaction with the latter and of sulfur oxide. and take a Rose coloring in the event of reduction. Examples:

Reaction of Schiff to highlight the Aldehyde S;
coloring with the periodic acid used in histology using the same principle.

The Fuchsine of schiff L

Used in the Coloring NOT (Periodic Acid Schiff) and the coloring of Feulgen. Its structure is presented in figure 2.

The Fuchsine of schiff C

It derives from Fuchsine Schiff L in the presence of functions aldehydes by restoration of the function quinone. Used in the Coloring NOT (Periodic Acid Schiff). Its structure is presented in figure 3.

Internal bonds

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