Fluorescent hybridization in-situ

See also: FISH

The fluorescent Hybridation in-situ (in Fluorescent English in situ hybridization or FISH) is a Technique of molecular biology of in situ Hybridation using probes marked using a marker fluorescent and used on cuts in microscopy.

These probes can be used on DNA or ARN (probe DNA), or on proteins (probe antibody).

The FISH is a technique of Cytogénétique making it possible to see elements inside the cell.

Used in medicine in association with the realization of a Chromosomic chart, it allows for example the search for microdélétions characteristic of the Syndrome of Williams or the Syndrome of Jacobsen. It makes it possible to differentiate a Syndrome CHARGES with a syndrome from Microdélétion 22q11, both resulting in cardiac problems.

Used in conjunction with other techniques of molecular biology (PCR, DGGE…), technique FISH makes it possible to make studies of biology the such study of the biodiversity of the species of the photosynthetic Picoplancton. In situ hybridization uses fluorescent probes recognizing of tax quite precise, for example a species, a kind or a class.

An alternative of the technique is called CARD-FISH for catalyzed to defer deposition in situ fluorescent hybridization .

Related article

See too

  • Marta de Menezes, a Portuguese artist realizing of films using the technique of the FISH.

Random links:Fay-of-Brittany | A tragedy at Mr. Grassot | Bank of the Guadeloupe | Pyruvate kinase | Beautiful infidels | Ngöbe-Buglé