Esculine (gélose)

The Esculine (gélose) is a Culture medium to study the Hydrolyze esculine. The test of the esculinase is an important criterion for an orientation towards Enterococcus

Composition

* Peptone: .......................... 10,0 G * Esculine: .......................... 1,0 G iron *citrate Ammonia Al: ......... 1,0 G * agar: ................... 20,0 G * pH = 7,4

Preparation

45 G per liter. Traditional pressure-sealing.

Test of the esculinase

The esculine is a formed heteroside of a complex glucid which can be degraded by the esculinase. The products of the reaction are the Glucose and the Esculétine. The formed esculétine can react with the ions Fer III to give a black precipitate.

Reading

  • If the medium is black: iron III reacted with the esculétine; the bacterium has the enzyme esculinase

  • If the medium remains gray, it did not have reaction there; the bacterium does not have the enzyme

to see too

  • Culture media (list)

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