Denaturation of the DNA
The rupture of the Hydrogen bonds between the nitrogenized Bases of the two chains complementary to the DNA involves the separation of the two bits: it is the phenomenon of denaturation.
This denaturation can be realized In vitro by subjecting the DNA to any chemical agent or physical able to destabilize the hydrogen bonds, like the pH, the temperature, certain solvents, of the high ionic concentrations, of the alkaline agents,…
Contrary to the denaturation of the Proteins, that of the DNA is perfectly reversible, at the time of a sufficiently slow return to the initial conditions: the bases are réapparient naturally.
The denaturation can be easily followed by Spectroscopie to 260 Nm from the existence of a Effet hyperchrome. The coefficient absorption ε of the molecule is increased by a factor approximately 1,4 during the transition doubles bit → simple bit.
| Random links: | Antz | Zygaeninae | The Man with the orchis | Alain Louvier | Marc Burns | County of Wuchuan | Oecophoridae |