DCL (gélose) (Désoxycholate-Citrate-Lactose) (medium of Hynes)

DCL (Gélose) (Désoxycholate- Citrate - Lactose) (medium of Hynes) is a Culture medium

Use

Insulation of the Salmonella - Shigella

Composition

  • Peptone 5,0 grams

  • Beef extract 5,0 grams
  • Lactose 10,0 grams
  • Citrate of Sodium 8,5 grams
  • Citrate of iron III 1,0 gram
  • Désoxycholate of Sodium 5,0 grams
  • Red neutral 0,020 gram
  • Thiosulfate of sodium 5,4 grams
  • Agar-agar 12,0 grams
  • pH = 7,3

Preparation

52 grams by liter.NE NOT AUTOCLAVER.

Reading

The colonies are colonies of bacillus S Gram -.

  • red Colonies: Lactose +
  • colorless Colonies or yellows: lactose -
  • Colonies in black center: H2S +
  • Colonies without black center: H2S -

to see too

  • Culture media (list)

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