Désoxycholate-Citrate-Lactose-Saccharose
Désoxycholate-Citrate-Lactose-Saccharose is a Culture medium.
Use
Insulation of the Salmonella - Shigella _ No the invasion of the medium by proteus
Composition
- Peptone (special mixture) 10,0 grams
- Lactose 5,0 grams
- Saccharose 5,0 grams
- Citrate of Sodium 10,5 grams
- Désoxycholate of sodium 2,5 grams
- Red neutral 0,03 grams
- Thiosulfate of sodium 5,0 grams
- agar 12,0 grams
- pH = 7,3
Preparation
50 gram by liter.NE NOT AUTOCLAVER.
Reading
The colonies are colonies of bacilli Gram-
- . Red colonies: lactose +
- colorless Colonies or yellows: lactose -
- if the composition includes iron III a black center reveals the production of Sulfure of hydrogen (H2S+)
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Culture media (list)