CLED (gélose)

Use

insulation or numeration of the urines (CLED = Cystine defective Electrolyt Lactose)

Composition

  • peptone 4,0 G

  • beef extract 3,0 G
  • peptic peptone of meat 4,0 G
  • L-cystine 0,128 G
  • lactose 10,0 G
  • blue of bromothymol 0,02 G
  • agar 13,0 G
  • pH = 7,3

Preparation

34,2 G per liter. Traditional sterilization.

Reading

  • yellow Colonies: lactose +

  • blue or green Colonies: lactose -

The low content of ions makes it possible to avoid invasion by Proteus .

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