Cétrimide (Gélose with)
Use
Insulation of Pseudomonas aeruginosa
Composition
- peptone of Gelatin: ........................ 16,0 G - peptone of Casein: ......................... 10,0 G - Bromide of tétradonium (cétrimide): .......... 0,2 G - Acid nalidixic: .......................... 15,0 Mg - potassium sulfate: ....................... 10,0 G - Chloride magnesium ........................ 1,4 G - agar: ....................................... 10,0 G- pH = 7,1
Role of some components:
inhibiter cétrimide and acid nalidixic stimulation of the production of pyocianine (coloring blue): potassiumsulfate and magnesium chloride Preparation
24,2 G per liter. In certain compositions the cétrimide is present before pressure-sealing. In others he is added with the acid nalidixic which is sometimes absent.
Use
The gélose with the cétrimide is a medium allowing the selective insulation of Pseudomonas aeruginosa in varied products. However, Serratia, Klebsiella and Acinetobacter can cultivate there. After sowing, the medium is thus incubated with 42°C during 24:00 before being examined, which makes it possible to improve selective insulation of Pseudomonas aeruginosa.Reading
Obtaining colonies having a blue or blue-green characteristic pigmentation and a fluorescence under ultra-violets to 254 Nm directs towards Pseudomonas aeruginosa.to see too
- Culture medium
- Culture media (list)
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