BEA (gélose Bile Esculine Azide)

See also: BEA

Use

Selective insulation of the Enterococcus and Streptococcus of the group D: the Azoture of sodium prevents the growth of the Gram bacteria - and of all the streptocoques ones except those of the group D, and the ox bile inhibits the growth of bacteria Gram-plus.

Composition

according to the ISO standard 7899-2:

- Peptone: ................................... 17,0 G - Peptic meat Peptone: ................. 3,0 G - Yeast Extract: .......................... 5,0 G - Esculine: .................................. 1,0 G - Citrate of sodium: .......................... 1,0 G - Ammoniacal iron Citrate: .................. 0,5 G - Ox Bile dehydrated: .................. 10,0 G - Azide of sodium: ....................... 0,25 G - Sodium chloride: ......................... 5,0 G - Agar: ...................................... 13,0 G

pH = 7,1

Preparation

45 G per liter. Traditional pressure-sealing

Reading

Black colonies show the hydrolysis of the esculine revealed by the iron III ions (a confusion is possible with the Sulfure of hydrogen but the bacteria cultivating on this medium are usually H2S -).

Certain manufacturers propose a similar medium without azide (gélose Bile-Esculine).

to see too

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