A automatic pipette or micropipette or automatic pipettor is a system of pipettage of precision used inter alia in analytical Chimie and Molecular biology. Among the marks, let us quote Pipetman® (registered trademark of Gilson-Rainin), Finnpipet® , etc

Description

They are systems actuating of the Piston S. the modification length of the airstream inside the apparatus makes it possible to aspire or drive out volumes of liquids with a high degree of accuracy.

A serrated roller makes it possible the user to regulate volume to be taken, using an indicator with figures.

There exists generally a range of models according to volume with pipetter and the precision of the taking away to be carried out:

  • P5000: pipetter to 5 ml of solution allows.
  • P1000: pipetter from 200 allows to 1000 µl of solution.
  • P200: pipetter from 20 allows to 200 µl
  • P20: pipetter from 10 to 20 µl
  • P10 allows: pipetter from 0.5 to 10 µl allows.

Points

It is necessary to have ends (or " Point s" or " Cone s") who are adapted to the micropipette used. These ends must be the most frayed possible, in particular for small volumes, in order to better see taken volume. They are generally laid out in limp stérilisables, provided with a bored plate, where they are put.

There exist ends provided with a cotton filter. They make it possible to avoid the contamination being able to come from the figure of the pipette or the airstream.

There exist also boxes out of sterile package already filled of ends. These boxes are Jetables.

One takes an end simply by applying the end of the micropipette in the collar of the end. While supporting a little, the end is inserted and been able to be withdrawn of the box.

An ejector of cones makes it possible to throw the soiled end of the micropipette without making use of its hands.

Use

One starts by regulating volume to be taken.

For pipetter, one holds the automatic pipette by the body with the four fingers of the right hand (if one is droitier) and the inch is used to actuate the piston or the ejector of cones.

One makes use of the left hand to hold, open or close the Microtube S.

Adjustment

  • a small key makes it possible to tighten or loosen a screw modifying the length of the airstream and makes it possible to regulate the micropipette.

Manufacturers

  • BioHit
  • Eppendorf
  • Finnpipette, with the cones " Finntips".
  • Gilson-Rainin
  • Labsystems
  • …

Micropipettes multichannel

Allow to carry out 8 or 12 taking away of a blow more quickly to fill a Plaque 96 wells or a Plaque of microdilutions. The solution to be distributed on the plate is versed in small a Auge out of disposable sterile plastic.

Electronic Micropipette

The sleeve of the micropipette contains an electrical motor which carries out swabbing. Buttons make it possible to carry out programming using screen with liquid crystals. A large button makes it possible to launch the entered program of pipettage. The rest-pipette is connected to the sector and is used as a basis of recharging of the battery of the apparatus.

See too

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