Antisens
The molecules antisens interact with the complementary bit of nucleic acid, modifying the expression of the Gène S.
Certain areas of DNA code Gènes, which are of the instructions specifying the order of the Amino-acid in a Protéine, but have also regulating sequences, sites of épissage, not-coding Introns. So that a cell uses information, a bit of the DNA is used as model for the synthesis of a bit complementary to ARN. The bit of DNA being used as model corresponds to the bit transcript with a sequence antisens and the ARN produced messenger has a sequence " sens" (complementary to the bit antisens). Because the DNA is double bit, the bit complementary to the sequence antisens is label like the bit non-transcript and has the same sequence as the ARN messenger except that the thymines of the DNA are substituted by bases uracils in the ARN).
Bit of DNA 1: direction
Bit of DNA 2: antisense (transcipt in) → bit of ARN (direction)
Several forms of antisens were developed and can be categorized overall in antisens depend on an enzyme or antisens with steric blocking.
The antisens dependant on an enzyme include the forms dependant on the activity of RNAse H to degrade the ARN messenger targets, as well as the DNA simple bit, the ARN and the antisens phosphorothioate. The system hok/hok (plamide R1) is a example process of regulation of the ARN messenger antisens by the enzymatic degradation of duplex ARN: Resulting ARN. The ARN doubles bit acts like antisens dependant on an enzyme by the ARNi/ARNsi way, implying the recognition of the target ARN messenger by the pairing of the bits direction-antisens followed by the degradation of the target ARN messenger by the RNA-induced silencing complex (RISC).
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